Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acid-sensing pathways of rat duodenum.

Y Akiba1, P H Guth, E Engel

  • 1CURE: Digestive Diseases Research Center, Los Angeles, California 90073, USA.

The American Journal of Physiology
|August 13, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BIoecology of Oncideres cervina Thomson (Coleoptera: Cerambycidae) on Persea americana Mill. (Lauraceae).

Brazilian journal of biology = Revista brasleira de biologia·2023
Same author

Spatial distribution and sample size to estimate Lycosa erythrognatha (Araneae: Lycosidae) population density overwintering.

Brazilian journal of biology = Revista brasleira de biologia·2020
Same author

Relationship Between Stink Bug Populations in Winter Shelters and Atmospheric Variables in Soybean Growing Areas in Southern Brazil.

Neotropical entomology·2020
Same author

A novel hybrid nanofibrous strategy to target progenitor cells for cost-effective in situ angiogenesis.

Journal of materials chemistry. B·2020
Same author

Forage system is the key driver of mountain milk specificity.

Journal of dairy science·2019
Same author

Gastric carbonic anhydrase IX deficiency: At base, it is all about acid.

Acta physiologica (Oxford, England)·2018

Luminal acid stimulates capsaicin-sensitive nerves in the rat duodenum, triggering vasodilation through calcitonin gene-related peptide (CGRP) and nitric oxide (NO) pathways. This response is mediated by vanilloid receptors.

Area of Science:

  • Gastroenterology
  • Neurogastroenterology
  • Vascular Physiology

Background:

  • The duodenal hyperemic response to acid is a critical protective mechanism.
  • Capsaicin-sensitive afferent nerves and their released mediators are implicated in regulating duodenal blood flow.

Purpose of the Study:

  • To investigate the role of capsaicin-sensitive nerves and specific signaling pathways in mediating the duodenal hyperemic response to luminal acid.
  • To determine if acid acts as an endogenous ligand for duodenal vanilloid receptors.

Main Methods:

  • Utilized Laser-Doppler flowmetry to measure duodenal blood flow in urethane-anesthetized rats.
  • Superfused duodenal mucosa with solutions of varying pH, capsaicin, bradykinin, and specific receptor antagonists or inhibitors (vanilloid receptor antagonists, CGRP receptor antagonist, NO synthase inhibitor, cyclooxygenase inhibitor).

Related Experiment Videos

  • Performed selective ablation of capsaicin-sensitive nerves.

    • Main Results:

    • Capsazepine, a selective vanilloid receptor antagonist, dose-dependently inhibited acid- and capsaicin-induced hyperemia but not bradykinin-induced hyperemia.
    • Inhibition of acid-induced hyperemia was observed with CGRP-(8-37) and N(G)-nitro-L-arginine methyl ester (a nitric oxide synthase inhibitor), but not with indomethacin (a cyclooxygenase inhibitor).
    • Selective ablation of capsaicin-sensitive nerves also reduced the hyperemic response to acid.

    Conclusions:

    • Luminal acid stimulates vanilloid receptors on capsaicin-sensitive afferent nerves in the rat duodenum.
    • Activation of these nerves leads to vasodilation mediated by the calcitonin gene-related peptide (CGRP) and nitric oxide (NO) pathways.
    • Acid is identified as a potential endogenous ligand for duodenal vanilloid receptors, distinct from its effect on bradykinin-induced responses.