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Related Experiment Videos

CD69 surface expression on human lung eosinophils after segmental allergen provocation.

P Julius1, W Luttmann, B Knoechel

  • 1Dept. of Pneumology, University Medicar Clinic, Freiburg, Germany.

The European Respiratory Journal
|August 13, 1999
PubMed
Summary

Allergen challenge in asthma significantly increases CD69 expression on eosinophils 18 hours later. This upregulation of eosinophil activation is linked to elevated granulocyte-macrophage colony-stimulating factor (GM-CSF) levels.

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Symbols, abbreviations and units.

The European respiratory journal·2014

Area of Science:

  • Immunology
  • Respiratory Medicine
  • Allergy Research

Background:

  • CD69 expression on eosinophils is a known marker of activation in asthma.
  • The precise role of allergens in regulating eosinophil CD69 expression in vivo is not fully understood.

Purpose of the Study:

  • To investigate whether allergen provocation in vivo can induce CD69 expression on eosinophils.
  • To explore the relationship between allergen-induced CD69 expression and inflammatory mediators.

Main Methods:

  • Ten allergic asthmatic patients underwent segmental allergen provocation.
  • CD69 expression on bronchoalveolar lavage (BAL) eosinophils was measured by flow cytometry.
  • Interleukin concentrations, including GM-CSF, were analyzed in BAL fluid using ELISA.

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Main Results:

  • CD69 expression on eosinophils did not significantly differ 10 minutes after allergen or saline challenge.
  • A significant increase in CD69 expression on eosinophils was observed 18 hours post-allergen challenge (p<0.03).
  • Elevated granulocyte-macrophage colony-stimulating factor (GM-CSF) concentrations (p<0.05) and a correlation between CD69 expression and GM-CSF (r=0.7, p<0.025) were noted 18 hours after allergen provocation.

Conclusions:

  • Allergen provocation in allergic asthma leads to an allergen-dependent, endobronchial upregulation of eosinophil activation.
  • CD69 expression serves as a valuable indicator of this allergen-induced eosinophil activation.
  • The findings highlight the role of GM-CSF in the inflammatory cascade following allergen challenge in asthma.