Osteocalcin-immunoreactive primary sensory neurons in the rat spinal and trigeminal nervous systems

Osteocalcin-immunoreactivity (OC-ir) was examined in spinal and trigeminal primary sensory neurons of the adult rat. Sixteen percent of dorsal root ganglion (DRG) neurons were immunoreactive (ir) for this protein. These neurons were mostly large and measured 594-4583 microm(2) (mean+/-S.D.=2243+/-748 microm(2)). Thirty-four percent of DRG neurons >1200 microm(2) and 4% of those in the range 600-1200 microm(2) showed the ir. Virtually all DRG neurons <600 microm(2) were devoid of OC-ir. In the trigeminal ganglion (TG), 25% of neurons exhibited the ir. Such neurons were of various sizes (range=156-2825 microm(2), mean+/-S.D.=1234+/-543 microm(2)). Forty-five percent of TG neurons >800 microm(2) and 6% of those <400 microm(2) were immunoreactive for this protein. Twelve percent of TG neurons in the range 400-800 microm(2) showed the ir. In the mesencephalic trigeminal tract nucleus (Mes5), 63% of primary sensory neurons contained OC-ir. Virtually all OC-ir DRG and Mes5 neurons co-expressed parvalbumin-ir but not CGRP-ir. On the other hand, only 31% of OC-ir neurons co-expressed parvalbumin-ir and 10% co-expressed CGRP-ir in the TG. The present study indicates that DRG and Mes5 primary sensory neurons co-expressing OC- and parvalbumin-irs are spinal and trigeminal proprioceptors. OC-ir TG neurons which co-express parvalbumin- and CGRP-irs appear to include low-threshold mechanoreceptors and nociceptors, respectively.