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sst5 somatostatin receptor mRNA induction by mitogenic activation of human T-lymphocytes
C E Ghamrawy1, C Rabourdin-Combe, S Krantic
1Laboratoire de Biologie Cellulaire et Moléculaire, UMR 49, Ecole Normale Supérieure, Lyon, France.
Abstract:
SRIF has neuro-immunomodulatory actions on immune cells, including T-lymphocytes. Molecular mechanisms involved in these actions were studied by RT-PCR analysis of SRIF receptor expression in resting and initogen-activated human T-lymphocytes. Our results point to the mitogen-associated induction of sst5 receptor subtype. Conversely, sst3 receptor appears constitutively expressed in both activity states. Assessment of biologic actions of SRIF14 in activated T-lymphocytes indicates that, in nanomolar concentration range, this peptide moderately inhibits mitogen-induced IL-2 secretion. Nevertheless, T-lymphocyte proliferation is not inhibited in the presence of SRIF14 but is even slightly increased. Altogether these data suggest a complex mechanism of SRIF neuro-immunomodulatory actions.