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Hemolysin of Myxococcus fulvus NK 35 i. production and isolation
Abstract:
Myxococcus fulvus NK 35 has been shown to produce a soluble hemolysin which lysed rabbit, human, horse, and sheep erythrocytes. A medium (Varghese's medium) was devised in which a maximum of hemolysin was produced in 6 days at 28 degrees C under static conditions. The lysin was precipitated by complete saturation of the culture filtrate with ammonium sulphate, followed by dialysis against saline. Other enzyme systems were destroyed by heating at 100 degrees C. Further purification was achieved by passing through a Sephadex G-25 column, giving a single peak with 0.01 M of phosphate buffer, pH 6.6. Like the hemolysin of Pseudomonas aeruginosa, this hemolysin is non-proteinic and withstands 100 degrees C for 30 minutes.
Insights
Myxococcus fulvus NK 35 produces a soluble hemolysin effective against various erythrocytes. This non-proteinic hemolysin is stable at 100°C and can be purified using ammonium sulfate precipitation and Sephadex G-25 chromatography.
Area of Science:
- Microbiology
- Biochemistry
Background:
- Myxococcus fulvus NK 35 produces a soluble hemolysin.
- This hemolysin lyses erythrocytes from rabbits, humans, horses, and sheep.
Purpose of the Study:
- To devise an optimal medium for hemolysin production.
- To purify and characterize the hemolysin.
Main Methods:
- Cultivation in Varghese's medium at 28°C for 6 days.
- Purification via ammonium sulfate precipitation, dialysis, and Sephadex G-25 chromatography.
- Heat stability testing at 100°C.
Main Results:
- Maximum hemolysin production achieved in 6 days under static conditions.
- Purified hemolysin obtained as a single peak.
- Hemolysin demonstrated non-proteinic nature and heat stability up to 100°C for 30 minutes.
Conclusions:
- Varghese's medium supports optimal hemolysin production.
- The hemolysin is non-proteinic and heat-stable, similar to Pseudomonas aeruginosa hemolysin.