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Related Experiment Videos

Proteins can adopt totally different folded conformations.

G Damaschun1, H Damaschun, K Gast

  • 1Humboldt-Universität zu Berlin, Institut für Biologie, c/o Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Strasse 10, Berlin, PF 740238, D-13092, Germany. gdamasc@mdc-berlin.de

Journal of Molecular Biology
|August 17, 1999
PubMed
Summary

Protein structure is influenced by its environment. Yeast phosphoglycerate kinase (PGK) misfolds into more ordered states, like alpha-helices or amyloid-like fibers, under specific conditions.

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Area of Science:

  • Biochemistry
  • Structural Biology
  • Protein Folding

Background:

  • Protein three-dimensional structure is crucial for function.
  • Environmental interactions significantly impact protein conformation.
  • Yeast phosphoglycerate kinase (PGK) serves as a model system.

Purpose of the Study:

  • To investigate the influence of environmental factors on protein structure.
  • To characterize the non-natively folded states of PGK.
  • To understand the role of acid-unfolded states in protein misfolding.

Main Methods:

  • Analysis of protein secondary structure (alpha-helices, beta-sheets).
  • Induction of protein unfolding using acidic pH and low ionic strength.
  • Induction of misfolding using trifluoroethanol and anions (chloride, trichloroacetate).

Related Experiment Videos

  • Characterization of protein conformation and fiber formation.

    • Main Results:

    • Acidic pH and low ionic strength induce random-walk structures in PGK.
    • Trifluoroethanol transforms acid-unfolded PGK into a flexible alpha-helix.
    • Anions induce amyloid-like fiber formation with extensive beta-sheet structures.
    • Non-natively folded states exhibit higher secondary structure content than native PGK.
    • Misfolding originates from an expanded, acid-unfolded state.

    Conclusions:

    • Environmental conditions dramatically alter protein structure.
    • PGK can adopt highly ordered, non-native conformations.
    • Acid-unfolded states are key intermediates in PGK misfolding pathways.