Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Metabolic engineering with recombinant adenoviruses.

P A Antinozzi1, H K Berman, R M O'Doherty

  • 1Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA.

Annual Review of Nutrition
|August 17, 1999
PubMed
Summary

Recombinant adenoviral vectors enable gene delivery for studying metabolic regulation, offering insights into obesity and diabetes by exploring gene function and hormone levels.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive tumor-agnostic evaluation of genomic and epigenomic-based approaches for the identification of circulating tumor DNA in early-stage breast cancer.

ESMO open·2025
Same author

Comparison of proteomic and metabolomic profiles of mutants of the mitochondrial respiratory chain in Caenorhabditis elegans.

Mitochondrion·2014
Same author

Isoprenylcysteine carboxylmethyltransferase regulates mitochondrial respiration and cancer cell metabolism.

Oncogene·2014
Same author

Branched-chain amino acid levels are associated with improvement in insulin resistance with weight loss.

Diabetologia·2011
Same author

Composition and energy harvesting capacity of the gut microbiota: relationship to diet, obesity and time in mouse models.

Gut·2010
Same author

Insulin resistance is associated with a metabolic profile of altered protein metabolism in Chinese and Asian-Indian men.

Diabetologia·2010

Area of Science:

  • Metabolic regulation and gene therapy
  • Mammalian physiology and disease mechanisms

Background:

  • Metabolic homeostasis relies on complex tissue interactions, which are impaired in obesity and diabetes.
  • Developing novel molecular and pharmacologic strategies is crucial for reversing metabolic disorders.

Purpose of the Study:

  • To review the application of recombinant adenoviral vectors in metabolic research.
  • To explore the use of viral gene transfer for understanding gene function and metabolic regulation.

Main Methods:

  • Utilizing recombinant adenoviral vectors for gene delivery to cell cultures and animal tissues.
  • Investigating gene function in glucose-stimulated insulin secretion and glycogen metabolism.
  • Evaluating candidate genes involved in metabolic dysfunction.

Related Experiment Videos

Main Results:

  • Adenoviral vectors provide insights into biochemical mechanisms regulating insulin secretion.
  • They aid in understanding the function of newly discovered genes in glycogen metabolism.
  • Viral vectors facilitate the evaluation of candidate genes like glucose-6-phosphatase.

Conclusions:

  • Recombinant adenoviral vectors are powerful tools for dissecting metabolic regulatory mechanisms.
  • Viral gene transfer enhances the understanding of genes contributing to metabolic disorders.
  • This approach aids in developing strategies for metabolic disease treatment.