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In vitro selection of nucleic acids and proteins: What are we learning?
1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA. rroberts@its.caltech.edu
Current Opinion in Structural Biology
|August 17, 1999
Summary
In vitro selection experiments isolate functional nucleic acids, peptides, and proteins. These methods offer a powerful alternative to rational design for creating novel biopolymers with specific properties.
Area of Science:
- Biochemistry and Molecular Biology
- Synthetic Biology
Background:
- In vitro selection has been a key technique for nearly a decade.
- It has reshaped understanding of molecular mimicry and catalysis.
- This approach is effective for generating biopolymers with desired functions.
Purpose of the Study:
- To review the advancements and impact of in vitro selection.
- To highlight how new methods enhance functional strategies.
- To discuss challenges for rational design in light of selection successes.
Main Methods:
- In vitro selection experiments to isolate functional molecules.
- Expansion of library size for increased diversity.
- Facilitation of recombination for positive mutations.
Main Results:
- Successful isolation of novel nucleic acids, peptides, and proteins.
- Demonstration of in vitro selection's efficacy over rational design for specific properties.
- Advancements in methods mimicking natural evolutionary strategies.
Conclusions:
- In vitro selection is a powerful tool for discovering functional biopolymers.
- New methods enhance the capabilities of functional selection strategies.
- Structural insights into selected molecules pose future challenges for rational design approaches.
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