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Mapping and complex expression pattern of the human NPAP60L nucleoporin gene
V Trichet1, D Shkolny, I Dunham
1Department of Biological Sciences, University of Alberta, Edmonton, Canada.
Cytogenetics and Cell Genetics
|August 18, 1999
Summary
Researchers identified NPAP60L, the human version of a rat nuclear pore-associated protein gene. Its complex expression in humans suggests varied roles, with potential conservation in male germ cells.
Area of Science:
- Genetics
- Molecular Biology
- Human Chromosome Research
Background:
- The rat nuclear pore-associated protein gene, Npap60, has a known function.
- Human chromosome 22q13.3 is a region of interest for gene identification.
Purpose of the Study:
- To identify the human homolog of the rat Npap60 gene.
- To characterize the expression and genomic organization of the identified human gene.
- To investigate potential conserved functions.
Main Methods:
- Clone mapping to human chromosome 22q13.3.
- Northern analysis for gene expression.
- Somatic cell hybrid mapping for gene localization.
Main Results:
- Identified NPAP60L as the human homolog of rat Npap60.
- Observed a more complex expression pattern for human NPAP60L compared to its rat counterpart.
- Detected at least three alternate 3' ends and possible alternate 5' ends in NPAP60L.
- Located additional NPAP60L-related copies on human chromosomes 5, 6, and 14.
Conclusions:
- Human NPAP60L exhibits complex gene expression, suggesting diverse functions.
- A conserved role for NPAP60L in male germ cells is suggested by one transcript.
- The presence of multiple related copies indicates potential gene duplication or pseudogenes.