Related Experiment Videos
Development and validation of an assay for urinary tissue factor activity.
B A Lwaleed1, M Chisholm, J L Francis
1University Department of Haematology, Southampton University Hospitals, UK.
Journal of Clinical Pathology
|August 18, 1999
Summary
Urinary tissue factor (uTF) can indicate monocyte/macrophage activation. This sensitive and specific assay may aid in diagnosing coagulation disorders in cancer and inflammatory diseases.
Area of Science:
- Biochemistry
- Hematology
- Oncology
Background:
- Coagulation activation is common in cancer and inflammation.
- Tissue factor (TF) from monocytes/macrophages initiates coagulation.
- TF's role in disease necessitates diagnostic markers.
Purpose of the Study:
- To assess urinary tissue factor (uTF) as a marker of monocyte/macrophage activation.
- To determine the diagnostic utility of uTF in disease states.
Main Methods:
- Urine samples were ultracentrifuged to isolate TF-containing vesicles.
- TF was solubilized and quantified using a chromogenic assay.
- Assay was optimized for microtitre plates.
Main Results:
- The uTF assay demonstrated high sensitivity, specificity, and reproducibility.
- Normal uTF levels exhibited a narrow range, unaffected by age, sex, or smoking.
- Sample storage and presence of blood did not significantly impact uTF levels.
Conclusions:
- The developed uTF assay shows potential for diagnosing hemostatic activation.
- This method could be valuable in managing patients with cancer and inflammatory conditions.