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Updated: Oct 19, 2026

Mechanical Stimulation of Chondrocyte-agarose Hydrogels
Published on: October 27, 2012
Effect of hydrogen peroxide on the metabolism of articular chondrocytes
1Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka-sayama, Osaka, Japan.
Objective:
To examine the effect of hydrogen peroxide on chondrocyte metabolism.
Materials And Methods:
Bovine articular chondrocytes were used. Proteoglycan (PG) synthesis was measured with [35S] sulfate incorporation. For detection of apoptosis, the TdT-mediated dUTP-biotin nick end labeling (TUNEL) and annexin V assay were used. Extracellular-regulated protein kinase (ERK) activity was measured using a mitogen-activated protein kinase assay system.
Results:
Addition of hydrogen peroxide resulted in the inhibition of PG synthesis, apoptosis, and enhanced ERK activity.
Conclusion:
Hydrogen peroxide plays an important role in regulating the metabolism of chondrocytes.
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