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Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
Published on: September 29, 2011
Separation of double-stranded and single-stranded DNA in polymer solutions: I. Mobility and separation mechanism
1Abteilung Lehrach, Max-Planck-Institut für molekulare Genetik, Berlin-Dahlem, Germany. heller@mpimg-berlin-dahlem.mpg.de
Abstract:
We have studied the separation of single-stranded and double-stranded DNA in a matrix of entangled, linear poly-N,N-dimethylacrylamide. Our results give better insight into the mechanisms involved during separations in polymer solutions. The dependence of different parameters on DNA size, electric field, pore size and the polymer chain length are evaluated and compared to theoretical predictions. Striking differences between experimental data and predicted scaling laws are found. Our data should help to optimize DNA separation in capillary electrophoresis and to improve existing models for DNA separation in porous matrices.
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