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Updated: Oct 19, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
C García-Ruiz1, M C García, M Torre
1Centro de Tecnología de Alimentos y Servicios Biosanitarios, Universidad de Alcalá, Ctra. Madrid-Barcelona, Alcalá de Henares (Madrid), Spain.
Abstract:
Capillary electrophoresis was applied for the first time to determine soybean proteins in commercial soybean products. The most suitable conditions for the analysis of these products in less than 7 min were 0.05 M phosphate buffer (pH 8) with 1 M urea; detection wavelength, 254 nm; applied voltage, 20 kV; and temperature, 30 degrees C. Quantitation of soybean proteins was achieved using referenced conditions by means of the method of standard additions, using as standard a soybean protein isolate. This method was validated and applied to the quantitation of soybean proteins in commercial products derived from soybean protein isolate and soybean seeds.
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