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Updated: Jul 21, 2026
In vivo-like Organotypic Murine Retinal Wholemount Culture
Published on: January 12, 2010
[Cell culture of human lamina cribrosa in vitro]
Objective:
To investigate the physiology and pathology of the biosynthesis of extracellular matrix (ECM) by lamina cribrosa cells (LCC) and to establish a model of cultured LCC.
Methods:
The LCC of 10 human dead fetal eyes were cultured with enzyme digestion procedures in vitro. The cultured cells were identified by immunohistochemistry assays and electron microscopy.
Results:
The cultured cells were flat, polygonal in shape, and their cytoplasm contained abundant perinuclear granules. The nuclei were relatively transparent and nucleoli were clear, there were basement membrane-like material and ECM surrounding the cell membrane. The immunofluorescent staining was positive for collagen type I, III, IV, fibronectin and laminin, negative for glial fibrillar acid protein and factor-VIII. The characteristics mentioned above were coincident with the morphology of LCC. This cell line has been cultured to its fourth generation.
Conclusion:
We have successfully cultured a cell line of LCC, and it may be utilized in the study of pathogenesis of glaucoma.
