[Effects of PGMS on the functions of human platelets and endothelial cells]
Objective:
To study the effects of Propylene glycol mannte sulfate (PGMS), a heparin-like substance on the functions of platelets and endothelial cells.
Methods:
Using 125I labeled monoclonal antibody to measure the expression of GMP-140 on the surface of human platelets; and using naphthol blue black stain method to study the growth of human umbilical vein endothelial cells (HUVEC) and human umbilical vein smooth muscle cells (HUVSMG).
Results:
PGMS significantly inhibited thrombin stimulated expression of GMP-140 on the surface of human platelets. IC50 was 127 micrograms/ml. Indomethacin also inhibited GMP-140 expression, but its effect was weaker than that of PGMS with the IC50 of 502 micrograms/ml. PGMS did not affect the growth of HUVSMC. On the contrary, it significantly promoted the growth of HUVEC at very low concentration (1.6 micrograms/ml).
Conclusions:
PGMS showes anticoagulative effects on both platelets and endothelial cells and it might well be developed as a new antithrombotic and anticoagulateve agent.
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