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[Changes of PDGF-alpha and beta receptor gene expression in hypoxic rat pulmonary vessels]
Objective:
To reveal the role of platelet-derived growth factor-alpha and beta receptor in hypoxic pulmonary vascular remodeling. We investigated the changes of PDGF-alpha and beta receptor gene expression under hypoxic condition.
Methods:
Hypoxic pulmonary hypertension rats model was reproduced by normobaric hypoxia. Changes of pulmonary PDGF-alpha and beta mRNA were examined by in situ hybridization.
Results:
The PDGF-alpha receptor mRNA of the hypoxia group (3 days) declined to 86% compared with that of the control group, and shooting up to 217% of control at day 7, then sustained at 224% until day 14. (all P < 0.001). PDGF-beta receptor mRNA markedly increased at day 3 after hypoxia and maintained this increasing tendency consistently. Its top was 164% at day 14 (P < 0.001). The growth inhibitor, heparin had alleviated the increases of both rat pulmonary arterial pressure and PDGF receptor gene expression.
Conclusion:
Hypoxia led to the increase of PDGF-alpha and beta receptor gene expression in pulmonary vascular endothelial and smooth muscle cells, and might be involved in hypoxic pulmonary vascular remodeling.