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Published on: December 9, 2016
Effects of human platelet-derived growth factor-AB on sarcoma growth in vitro and in vivo
A Abdiu1, S Wingren, S E Larsson
1Department of Biomedicine and Surgery, Faculty of Health Sciences, University of Linköping, Sweden. avnab@mcb.liu.se
Abstract:
Platelet-derived growth factor (PDGF) has been proposed to play an important role in the growth of tumors. In order to study the effects of PDGF-AB on tumor growth in vivo, sarcoma-bearing mice were treated with PDGF-AB. The tumors, a malignant fibrous histiocytoma and an osteosarcoma, had functional PDGF receptors in vitro, as demonstrated by stimulation of PDGF-AB using a [3H]thymidine incorporation assay. Immunohistochemistry also revealed that both sarcoma xenografts expressed PDGF receptors. The tumor-bearing mice were given human PDGF-AB for 14 days, either continuously by an intraperitoneally placed mini-osmotic pump, or by daily injections. No effects on tumor growth in vivo were observed, as measured by tumor volume, autoradiography or cell cycle distribution. The histological appearance and ploidy of the tumors remained unaltered. The results indicate that, although the tumor cells are stimulated by PDGF-AB in vitro, the in vivo milieu or tumor growth pattern may render the tumors less susceptible to exogenously administered PDGF-AB in vivo.
Insights
Platelet-derived growth factor (PDGF) did not affect tumor growth in mice, despite in vitro stimulation. The in vivo environment may reduce tumor susceptibility to exogenous PDGF-AB, impacting cancer research.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Platelet-derived growth factor (PDGF) is implicated in tumor growth.
- PDGF signaling pathways are crucial in various cancers.
Purpose of the Study:
- To investigate the in vivo effects of PDGF-AB on sarcoma tumor growth.
- To determine if exogenous PDGF-AB administration influences tumor progression in a murine model.
Main Methods:
- Sarcoma-bearing mice were treated with human PDGF-AB for 14 days via continuous infusion or daily injections.
- Tumor growth was assessed by volume, autoradiography, and cell cycle distribution analysis.
- In vitro studies confirmed functional PDGF receptors on tumor cells using [3H]thymidine incorporation assays and immunohistochemistry.
Main Results:
- Exogenous PDGF-AB administration showed no significant effect on tumor volume or growth.
- Autoradiography and cell cycle distribution analyses did not reveal alterations in tumor proliferation.
- Histological appearance and ploidy of the tumors remained unchanged post-treatment.
Conclusions:
- Tumor cells expressing PDGF receptors are not necessarily responsive to exogenous PDGF-AB in vivo.
- The in vivo tumor microenvironment or growth dynamics may confer resistance to PDGF-AB.
- Further research is needed to understand the in vivo regulation of PDGF signaling in tumors.
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