Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inhibition of oncogene expression using vector-generated RNA antisense.

L Whitesell1, X Liu, R Bagatell

  • 1Department of Pediatrics, University of Arizona, Tucson, Arizona 85724, USA. whitelj@peds.arizona.edu

Methods (San Diego, Calif.)
|August 24, 1999
PubMed
Summary

Antisense RNA expression vectors offer a new way to study oncogenes in cancer. This paper details how to build and use these vectors, discussing their limitations in tumorigenesis research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toxicities of busulfan/melphalan versus carboplatin/etoposide/melphalan for high-dose chemotherapy with stem cell rescue for high-risk neuroblastoma.

Bone marrow transplantation·2016
Same author

fdg-pet in two cases of neurofibromatosis type 1 and atypical malignancies.

Current oncology (Toronto, Ont.)·2014
Same author

Inhibiting HSP90 to treat cancer: a strategy in evolution.

Current molecular medicine·2012
Same author

Antisense efficacy : site-restricted in vivo and ex vivo models.

Methods in molecular medicine·2011
Same author

Pharmacokinetically guided phase 1 trial of the IGF-1 receptor antagonist RG1507 in children with recurrent or refractory solid tumors.

Clinical cancer research : an official journal of the American Association for Cancer Research·2010
Same author

Destabilization of steroid receptors by heat shock protein 90-binding drugs: a ligand-independent approach to hormonal therapy of breast cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2001

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Expression Analysis

Background:

  • Antisense RNA expression vectors are novel tools for investigating oncogene roles in malignant transformation.
  • Understanding oncogene function is crucial for developing targeted cancer therapies.

Purpose of the Study:

  • To describe strategies for constructing antisense plasmid vectors using readily available reagents.
  • To outline techniques for introducing these vectors into cell lines and tumors.
  • To present methods for evaluating the biological effects of antisense RNA expression.

Main Methods:

  • Construction of antisense plasmid vectors from commercial reagents.
  • Introduction of vectors into target cells and tumors.
  • Evaluation of biological effects and assessment of limitations.

Related Experiment Videos

Main Results:

  • Detailed strategies for antisense plasmid vector construction are provided.
  • Effective methods for vector delivery into cells and tumors are presented.
  • Guidelines for evaluating biological outcomes and potential artifacts are discussed.

Conclusions:

  • Antisense RNA expression vectors are valuable for studying oncogene function in tumorigenesis.
  • The paper provides practical guidance on vector construction, delivery, and analysis.
  • Awareness of limitations and potential artifacts is essential for accurate interpretation of results.