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Mutations in the Saccharomyces cerevisiae gene SAC1 cause multiple drug sensitivity

W E Hughes1, M J Pocklington, E Orr

  • 1Department of Cell Biology, GlaxoWellcome, Medicines Research Centre, Gunnels Wood Road, Stevenage SG1 2NY, U.K. hughesw@icrf.icnet.uk

Yeast (Chichester, England)
|August 24, 1999
PubMed

Insights

Mutations in the SAC1 gene cause multiple drug sensitivity in yeast. This finding reveals a new role for Sac1p in cellular drug resistance and growth.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Cell Biology

Background:

  • Wild-type yeast (Saccharomyces cerevisiae) exhibit significant resistance to various drugs.
  • Previous work isolated novobiocin-sensitive mutants to study drug targets.
  • The mds1 mutant displayed sensitivity to multiple drugs, distinct from known mutants.

Purpose of the Study:

  • To characterize the genetic basis of the multiple drug-sensitive phenotype in the mds1 yeast mutant.
  • To investigate the role of the SAC1 gene in drug resistance.

Main Methods:

  • Genetic analysis of the mds1 mutant.
  • Identification and characterization of mutations in the SAC1 gene.
  • Phenotypic analysis of sac1 mutants for drug sensitivity.

Main Results:

  • The multiple drug sensitivity of mds1 is caused by mutations in the SAC1 gene.
  • Specific mutations within SAC1 were identified in the Mds1 strain.
  • Multiple drug sensitivity is a newly observed phenotype in some sac1 mutants.

Conclusions:

  • The SAC1 gene product, Sac1p, plays a role in yeast drug resistance.
  • Sac1p's functions in actin cytoskeleton, inositol metabolism, and ER transport may influence drug sensitivity.
  • A novel mechanism for Sac1p's involvement in drug resistance and cell growth is proposed.

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