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Comparison between collision induced dissociation of electrosprayed protonated peptides in the up-front source region
W D van Dongen1, J I van Wijk, B N Green
1Bijvoet Centre for Biomolecular Research, Department of Mass Spectrometry, Utrecht University, F.A.F.C Wentgebouw, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands. dongen@voeding.tno.nl
Abstract:
A systematic comparison between the up-front Collision induced dissociation (CID) mass spectra and low-energy CID tandem mass spectra from twenty-one singly and/or doubly charged peptides has been made. CID spectra of the peptides were recorded at different electrode voltages in the up-front source region of a single quadrupole instrument and different collision energies in the collision cell of a tandem quadrupole instrument. It was observed that up-front CID and low-energy CID yielded comparable product ion spectra from protonated peptides, and that the instrumental settings necessary for obtaining comparable CID mass spectra from the two methods are correlated.