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Related Experiment Videos

Improvement of EPA method 8330: complete separation using a two-phase approach.

M J Lang1, S E Burns

  • 1Chemistry Department, Western Michigan University, Kalamazoo 49008, USA.

Journal of Chromatography. A
|August 24, 1999
PubMed
Summary

A new chromatography method using a Bondesil CN/C18 column system fully separates 14 explosives in EPA Method 8330. This optimized approach enhances sample throughput by reducing analysis times.

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Area of Science:

  • Analytical Chemistry
  • Environmental Science

Background:

  • EPA Method 8330 is used for analyzing nitroaromatic and nitramine explosives.
  • Existing methods may require multiple analyses, impacting efficiency.

Purpose of the Study:

  • To develop an improved chromatographic method for separating 14 specific explosive analytes.
  • To enhance sample throughput and reduce analysis time compared to EPA Method 8330.

Main Methods:

  • Utilized a Bondesil CN guard column in series with a Bondesil C18 column.
  • Employed a 1:1 methanol:water mobile phase at a flow rate of 1.5 mL/min.
  • Optimized injection volume to 20 microliters to maintain resolution.

Main Results:

  • Achieved complete separation of all 14 nitroaromatic and nitramine analytes.

Related Experiment Videos

  • Demonstrated superior resolution with the specific CN/C18 column combination compared to others.
  • Identified methanol injection solvent as a factor affecting mobile phase composition and resolution.

    • Conclusions:

    • The developed two-phase chromatographic system offers a significant improvement over EPA Method 8330.
    • Single-run separation of 14 analytes increases sample throughput by decreasing overall analysis time.
    • The specific Bondesil C18 column is crucial for achieving the required resolution.