Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flow microsphere immunoassay-based method of virus quantitation.

T I Samoylova1, B F Smith

  • 1College of Veterinary Medicine, Auburn University, AL, USA.

Biotechniques
|August 24, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peptide phage display: opportunities for development of personalized anti-cancer strategies.

Anti-cancer agents in medicinal chemistry·2006
Same author

Infectivity enhancement for adenoviral transduction of canine osteosarcoma cells.

Gene therapy·2005
Same author

Molecular markers of glial tumors: current targeting strategies.

Current medicinal chemistry·2003
Same author

Haemolytic anaemia and exercise intolerance due to phosphofructokinase deficiency in related springer spaniels.

The Journal of small animal practice·2001
Same author

Utilization of umbilical cords to assess in utero exposure to persistent pesticides and polychlorinated biphenyls.

Journal of exposure analysis and environmental epidemiology·2001
Same author

A case-control assessment of risk factors for gallbladder carcinoma.

Digestive diseases and sciences·1999
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

A new flow microsphere immunoassay (FMIA) accurately quantifies adenovirus in vitro. This sensitive method offers a reliable alternative for adenovirus detection in various samples.

Area of Science:

  • Virology
  • Immunology
  • Analytical Chemistry

Background:

  • Adenovirus detection and quantitation are crucial for research and diagnostics.
  • Existing methods for adenovirus quantitation can be labor-intensive or lack sensitivity.

Purpose of the Study:

  • To develop a sensitive and accurate assay for adenovirus quantitation in vitro.
  • To validate a novel flow microsphere immunoassay (FMIA) for adenovirus detection.

Main Methods:

  • Developed a flow microsphere immunoassay (FMIA) using antibody-coated polystyrene microspheres.
  • Incubated microspheres with virus samples, stained with DNA-specific fluorescent dyes.
  • Quantitated virus-associated fluorescence using a flow cytometer.

Main Results:

Related Experiment Videos

  • The FMIA demonstrated high sensitivity for adenovirus detection.
  • Adenovirus adsorption to microspheres was optimized under various conditions.
  • The FMIA showed accuracy comparable to traditional 293 cell titering methods.

Conclusions:

  • The developed FMIA is a sensitive and accurate method for adenovirus quantitation.
  • This assay is suitable for quantifying adenovirus in viral stocks and biological samples.
  • FMIA offers a valuable tool for adenovirus research and diagnostics.