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Related Experiment Videos

Influence of sterilization on injectable bone biomaterials.

C Zahraoui1, P Sharrock

  • 1Laboratoire de Chimie Inorganique, Université Paul Sabatier, Toulouse, France.

Bone
|August 24, 1999
PubMed
Summary

Sterilization methods significantly impact injectable bone biomaterials. Copolylactic acid-modified calcium phosphate cements can be steam sterilized without degradation, offering promising bone regeneration properties.

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Area of Science:

  • Biomaterials Science
  • Orthopedic Surgery
  • Polymer Chemistry

Background:

  • Injectable bone cements are crucial in orthopedic surgery.
  • Common materials include acrylic bone cements, calcium phosphate cements, and novel composites.
  • Biopolymers like cellulose derivatives and chitosan are explored as modifiers.

Purpose of the Study:

  • To evaluate the effects of sterilization on the physico-chemical properties of novel polyester copolymer (copolylactic acid)-based bone biomaterials.
  • To assess the suitability of different sterilization techniques for injectable bone biomaterials.
  • To investigate the in vivo performance of modified calcium phosphate cements.

Main Methods:

  • Physico-chemical analysis of biomaterials after gamma, heat, and steam sterilization.
  • Capillary electrophoresis and gel exclusion chromatography were used to assess degradation and crosslinking.
  • In vivo implantation of calcium phosphate cement modified by copolylactic acid in a rabbit metaphyseal defect model.

Main Results:

  • Gamma sterilization degraded chitosan solutions and caused hydrolysis in aqueous copolylactic acid solutions.
  • Heat sterilization also degraded chitosan solutions, complicating ultrafiltration.
  • Apatite-copolylactic acid solids demonstrated stability after steam sterilization.
  • Ultrafiltration was used to prepare hydrolysis-free aqueous copolylactic acid solutions.
  • In vivo studies showed progressive bone tissue substitution and biomaterial resorption with mild inflammation.

Conclusions:

  • Sterilization methods critically affect the properties of injectable bone biomaterials.
  • Steam sterilization is suitable for apatite-copolylactic acid solids.
  • Copolylactic acid-modified calcium phosphate cements exhibit favorable bone regeneration potential.
  • Specialized sterilization methods are necessary for fragile biopolymers and reactive monomers in injectable forms.

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