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Platelet cryopreservation using a reduced dimethyl sulfoxide concentration and second-messenger effectors as
M L Lozano1, J Rivera, J Corral
1Unit of Hematology, Hemotherapy and Clinical Oncology, School of Medicine, Centro Regional de Hemodonación, Murcia, 30003, Spain.
Cryobiology
|August 25, 1999
Summary
Cryopreservation of therapeutic platelet concentrates (PCs) using reduced Me2SO and ThromboSol maintains platelet recovery and function, offering an alternative to standard 6% Me2SO cryopreservation.
Area of Science:
- Blood banking and transfusion medicine
- Cellular cryobiology
- Hematology
Background:
- Cryopreservation of platelet concentrates (PCs) is crucial for extending shelf life and improving inventory management.
- Current standard methods use 6% Me2SO, but alternatives are sought to optimize platelet preservation.
Purpose of the Study:
- To compare the efficacy of cryopreserving PCs using a novel method with reduced Me2SO (2%) plus ThromboSol against the standard 6% Me2SO method.
- To evaluate the impact of cryopreservation on platelet recovery, function, and surface marker expression.
Main Methods:
- Platelet concentrates were cryopreserved using either 6% Me2SO or 2% Me2SO with ThromboSol.
- Samples were stored at -80°C or -120°C for up to 3 months.
- Post-thaw analysis included cell recovery, aggregation, and binding of von Willebrand factor and fibrinogen.
Main Results:
- Cryopreservation with ThromboSol and 2% Me2SO yielded platelet recovery (90%) comparable to the standard 6% Me2SO method.
- Both methods resulted in increased P-selectin expression and moderate loss of GP Ibalpha.
- Platelet aggregation and vWF binding responses to ristocetin were similar between the two cryopreservation methods.
Conclusions:
- Cryopreservation of PCs using reduced Me2SO concentration combined with ThromboSol is a viable alternative to the conventional 6% Me2SO method.
- This alternative method preserves key in vitro functional characteristics of platelets.