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Unequal pronuclear size--a powerful predictor of embryonic chromosome anomalies
1Department of Obstetrics and Gynecology, Ramban Medical Center, Haifa, Israel.
Purpose:
Our purpose was to evaluate whether pronuclei of unequal size, observed in 13.7% of zygotes evaluated after in vitro fertilization (IVF), are predictive of chromosome anomalies in the developing embryo.
Methods:
Five ploidy of 38 embryos grown from zygotes with unequal-sized pronuclei was assessed by fluorescent in situ hybridization (FISH). Twenty-six embryos developed after intracytoplasmic injection of sperm (ICSI) and 12 embryos were derived from conventional IVF.
Results:
Chromosome anomalies were documented in the ICSI and IVF groups in 88.5 and 50% of cases, respectively.
Conclusions:
We suggest that FISH should be employed to examine the ploidy of zygotes with unequal pronuclei, prior to embryo transfer.