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Cyclic expression of mRNA transcripts for connective tissue components in the mouse ovary
S Oksjoki1, S Sallinen, E Vuorio
1Department of Molecular Biology and Medical Biochemistry, Turku University Central Hospital, University of Turku, Kiinamyllynkatu 4-6, FIN-20520 Turku, Finland.
Molecular Human Reproduction
|August 25, 1999
Summary
The ovarian extracellular matrix (ECM) actively participates in ovarian functions. Cyclic changes in ECM component production support follicular maturation, ovulation, and corpus luteum formation.
Area of Science:
- Reproductive Biology
- Cell Biology
- Connective Tissue Research
Background:
- Ovarian functions, including follicle development and corpus luteum formation, occur within a connective tissue matrix.
- The extracellular matrix (ECM) is a dynamic component of ovarian tissue.
Purpose of the Study:
- To investigate the active role of the ovarian extracellular matrix (ECM) in ovarian functions.
- To analyze the cyclic expression patterns of ECM components during the mouse oestrous cycle.
Main Methods:
- Quantitative analysis of mRNA levels for various ECM components and matrix-remodeling enzymes.
- Northern blot analysis was performed on mouse ovaries at six distinct stages of the 4-day oestrous cycle.
Main Results:
- Statistically significant cyclic expression patterns were observed for mRNAs encoding type III, IV, and VI collagens, biglycan, syndecan-1, and osteonectin.
- Cyclic changes in these structural ECM components were more pronounced than those for matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs).
Conclusions:
- The production and degradation of the ovarian ECM are integral to normal ovarian function.
- Cyclic ECM changes are associated with key events such as follicular maturation, ovulation, and corpus luteum formation.