Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Presynaptic mechanism for phorbol ester-induced synaptic potentiation.

T Hori1, Y Takai, T Takahashi

  • 1Department of Neurophysiology, University of Tokyo Faculty of Medicine, Tokyo 113-0033 Japan.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|August 25, 1999
PubMed
Summary

Phorbol ester enhances synaptic transmission by affecting protein kinase C and Doc2alpha-Munc13-1 interaction, independent of calcium influx. This study models presynaptic facilitation at the calyx of Held synapse.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A case of Cushing's disease associated with a non-functioning adrenal tumor].

Nihon Naibunpi Gakkai zasshi·1992
Same author

Effects of vasopressin and angiotensin II on neurones in the rat dorsal motor nucleus of the vagus, in vitro.

The Journal of physiology·1992
Same author

Electrophysiological study of neurotropin-induced responses in guinea pig hypothalamic neurons.

Brain research bulletin·1992
Same author

[Interhemispheric choroidal epithelial cyst associated with partial agenesis of the corpus callosum: case report and review of the literature].

No shinkei geka. Neurological surgery·1992
Same author

Polar glycosphingolipids in annelida. A novel series of glycosphingolipids containing choline phosphate from the earthworm, Pheretima hilgendorf.

The Journal of biological chemistry·1992
Same author

Pseudo-high affinity interleukin 2 (IL-2) receptor lacks the third component that is essential for functional IL-2 binding and signaling.

The Journal of experimental medicine·1992

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Synaptic Plasticity

Background:

  • Phorbol esters are known to facilitate neurotransmitter release at various synapses.
  • Phorbol ester-induced synaptic potentiation (PESP) serves as a valuable model for studying presynaptic facilitation.
  • Understanding the precise molecular mechanisms underlying PESP is crucial for insights into synaptic function.

Purpose of the Study:

  • To elucidate the molecular mechanisms responsible for phorbol ester-induced synaptic potentiation (PESP).
  • To investigate the roles of protein kinase C (PKC) and Doc2alpha-Munc13-1 interaction in PESP.
  • To determine if PESP is dependent on presynaptic calcium or potassium currents.

Main Methods:

  • Paired whole-cell recordings were performed on the calyx of Held and its postsynaptic target in rat brainstem slices.

Related Experiment Videos

  • The effects of phorbol ester on excitatory postsynaptic currents (EPSCs) were measured.
  • Presynaptic calcium and potassium currents were assessed.
  • The impact of protein kinase C inhibitors and a synthetic peptide targeting Doc2alpha-Munc13-1 interaction was evaluated.

    • Main Results:

    • Phorbol ester potentiated EPSCs without altering presynaptic calcium or potassium currents.
    • Inhibition of protein kinase C significantly attenuated PESP.
    • Presynaptic loading of a peptide mimicking Doc2alpha-Munc13-1 interaction also attenuated PESP, while a mutated peptide had no effect.

    Conclusions:

    • The presynaptic facilitatory effect of phorbol ester is downstream of calcium influx.
    • Protein kinase C activation and the interaction between Doc2alpha and Munc13-1 are key components of PESP.
    • These findings provide critical insights into the molecular regulation of presynaptic facilitation.