Backscattered electron imaging of titanium dioxide in frozen hydrated preparations
Related Experiment Video
Updated: Oct 19, 2026

09:01
High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
Published on: April 16, 2017
1L'Oreal Recherche, Department of Analytical Chemistry, 1 av. Eugene Schueller, BP22, 93601 Aulnay-sous-Bois Cedex, France.
Journal of Microscopy
|August 25, 1999
Abstract:
Backscattered electron (BSE) imaging was used to study ultrafine TiO2 crystals distribution in a test cream. The cream was fast frozen, cryofractured and observed uncoated at low temperature. The BSE detector was a microchannel plate. The results demonstrate that up-to-date photoprotective preparations can be investigated by this technique.
More Related Videos
Related Concept Videos
Electron Microscope Tomography and Single-particle Reconstruction
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Preparation of Samples for Electron Microscopy
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

