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Related Experiment Videos

Hyaluronidase ameliorates rejection-induced edema.

C Johnsson1, R Hällgren, A Elvin

  • 1Department of Transplantation Surgery, University Hospital, S-751 85 Uppsala, Sweden. Cecilia.Johnsson@kirurgi.uu.se

Transplant International : Official Journal of the European Society for Organ Transplantation
|August 26, 1999
PubMed
Summary

Hyaluronidase treatment reduced fluid buildup and improved organ transplant survival. This enzyme breaks down hyaluronan, a molecule that causes swelling in rejecting organs, offering a potential therapy for post-transplant edema.

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Area of Science:

  • Biochemistry
  • Immunology
  • Transplantation Medicine

Background:

  • Hyaluronan (HA), a glycosaminoglycan, accumulates in interstitial edematous tissue during organ transplant rejection.
  • This accumulation contributes to increased tissue water content and edema in rejecting allografts.

Purpose of the Study:

  • To investigate if hyaluronidase, an enzyme that degrades hyaluronan, can prevent or reduce edema and improve outcomes in a rat heart transplantation model.
  • To evaluate the efficacy of hyaluronidase therapy, alone or with cyclosporine, in managing allograft rejection-associated edema and graft survival.

Main Methods:

  • Heterotopic heart transplantations were performed between PVG and Wistar/Kyoto rats.
  • Recipient rats received prophylactic or therapeutic treatment with hyaluronidase, with or without cyclosporine.

Related Experiment Videos

  • Cardiac hyaluronan and water content were measured, and graft morphology and survival were assessed.

    • Main Results:

    • Daily intravenous hyaluronidase significantly reduced cardiac hyaluronan and water content by day six post-transplantation.
    • Hyaluronidase treatment led to better graft morphology and reduced mononuclear cell infiltration compared to controls.
    • Both prophylactic and therapeutic hyaluronidase, alone or combined with cyclosporine, prolonged graft survival times.
    • A two-hour hyaluronidase infusion also reduced established edema five days after transplantation.

    Conclusions:

    • Hyaluronan accumulation is critical in post-organ transplantation edema formation.
    • Hyaluronidase therapy is a viable strategy to reduce edema and potentially improve outcomes after organ transplantation.