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Structural and ultrastructural study of GH, PRL and SMT cells in male goat by immunocytochemical methods

M A Gómez1, B Garcés-Abadías, A Muñoz

  • 1Histología y Anatomía Patológica, Facultad de Veterinaria, Universidad de Murcia, España. magomez@fcu.um.es

Cells, Tissues, Organs
|August 26, 1999
PubMed

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Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.