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Random amplification of polymorphic DNA fingerprinting of Trypansoma evansi
S H Basagoudanavar1, J R Rao, R K Singh
1National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar.
Veterinary Research Communications
|August 26, 1999
Abstract:
The total genomic DNAs from Trypanosoma evansi isolates of bubaline, equine and cameline origin were amplified by polymerase chain reaction using eight arbitrarily selected 10-base primers. Informative band patterns were obtained for all isolates analysed. Depending upon the T. evansi isolate primer combination, between 1 and 11 reproducible DNA fingerprints of 205 to 3016 bp were amplified, suggesting minor and major differences in their RAPD (random amplified polymorphic DNA) profiles.