Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Regulation of complement factor H expression in L cells.

D P Vik1

  • 1Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, New Mexico, 87131-5276, USA. dvik@unm.edu

Biochemical and Biophysical Research Communications
|August 27, 1999
PubMed
Summary

Researchers identified an octamer sequence crucial for regulating the murine factor H gene

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Promoter activity of the 5' flanking region of the complement receptor type 1 (CR1) gene: basal and induced transcription.

Biochimica et biophysica acta·2000
Same author

Regulation of complement factor H in a human liver cell line by interferon-gamma.

Scandinavian journal of immunology·1999
Same author

Complement receptor type 1 gene regulation: retinoic acid and cytosine arabinoside increase CR1 expression.

Scandinavian journal of immunology·1999
Same author

Mechanism of regulation of complement receptor type 1 transcription by cytosine arabinoside in a pre-erythroid model.

Scandinavian journal of immunology·1999
Same author

Analysis of the promoter region of the murine complement factor H gene.

Biochimica et biophysica acta·1998
Same author

Characterization of the 5' flanking region of the human complement factor H gene.

Scandinavian journal of immunology·1997

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Mammalian Genetics

Background:

  • The factor H gene plays a critical role in regulating the complement system.
  • Understanding the basal promoter activity of the factor H gene is essential for comprehending its overall gene expression.
  • Fibroblast cell lines, such as L929 (L cells), are commonly used models for studying gene regulation.

Purpose of the Study:

  • To identify regulatory elements responsible for the basal promoter activity of the murine factor H gene.
  • To characterize the function of specific DNA regions in controlling factor H gene expression.
  • To elucidate the molecular mechanisms underlying factor H gene regulation in L cells.

Main Methods:

  • Luciferase reporter assays were employed to measure basal promoter activity.
  • Nested deletion constructs were utilized to map functional regions of the promoter.
  • Electrophoretic mobility shift assays (EMSAs) were performed to study protein-DNA interactions.
  • Sequence analysis was conducted to identify potential regulatory motifs.

Main Results:

  • A region between -811 and -344 of the murine factor H gene exhibited enhancer activity.
  • Further subdivision revealed two subfragments within this region, both possessing enhancer activity.
  • EMSAs demonstrated that these subfragments bind to a nuclear factor and can cross-inhibit each other's binding.
  • Sequence analysis identified an octamer motif present in both active subfragments.
  • A synthetic octamer oligomer successfully blocked nuclear factor binding in EMSAs.

Conclusions:

  • An octamer sequence within the -811 to -344 region is a key regulatory element for the murine factor H gene.
  • This octamer sequence plays a significant role in mediating basal expression of the factor H gene in L cells.
  • The findings provide insights into the transcriptional regulation of the factor H gene.

Related Experiment Videos