Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell proliferation in the developing human kidney.

T Nadasdy1, G Lajoie, Z Laszik

  • 1Department of Pathology, University of Oklahoma, Oklahoma City 73104, USA.

Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society
|August 27, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Exchange-bias and magnetic anisotropy fields in core-shell ferrite nanoparticles.

Scientific reports·2025
Same author

Serum and saliva immunoglobulin (immunoglobulin G, immunoglobulin A, and immunoglobulin M) dynamics in newborn calves and their association with health status during the first week of life: An exploratory study.

Journal of dairy science·2025
Same author

Interactions between pea protein and gellan gum for the development of plant-based structures.

International journal of biological macromolecules·2023
Same author

Koebner phenomenon with lichen planus in an area of previous vitiligo after COVID-19 vaccination and the creation of a locus minoris resistentiae.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Comment on 'Don't Judge a Book by its Cover. "Steroid Acne": an Unrecognized Role of Malassezia and Demodex?' by Melin et al.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Exchange-bias and magnetic anisotropy fields in core-shell ferrite nanoparticles.

Scientific reports·2021
Same journal

Early-Onset Severe Liver Fibrosis in a Toddler with Erythropoietic Protoporphyria Associated with Rare Compound Heterozygous FECH Variants.

Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·2026
Same journal

Fetiform Teratoma With a Digitiform Projection and Complex Organoid Differentiation (Adrenal and Pancreatic).

Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·2026
Same journal

Characterizing Fetal Hemorrhage in a Perinatal Autopsy Cohort.

Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·2026
Same journal

The Hepatic Histomorphology and Application of Ensemble Transfer Learning in Convolutional Neural Network Models in the Biopsies of Hepatic Langerhans Cell Histiocytosis.

Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·2026
Same journal

Drug Induced Liver Injury in Children: Practical Considerations for Pathologists.

Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·2026
Same journal

Childhood-Onset Desmin-Related Cardiomyopathy: Comprehensive Clinical, Histopathological, Immunohistochemical, Ultrastructural, and Molecular Characterization.

Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·2026
See all related articles

Cell proliferation in developing human kidneys was quantified using proliferating cell nuclear antigen (PCNA). Early tubules show high proliferation, while differentiated proximal tubules have low proliferation, indicating changes related to nephron segment differentiation.

Area of Science:

  • Nephrology
  • Developmental Biology
  • Cell Biology

Background:

  • Previous studies established low proliferation in adult and pediatric kidneys using proliferating cell nuclear antigen (PCNA).
  • Understanding cell proliferation during kidney development is crucial for comprehending nephrogenesis.

Purpose of the Study:

  • To quantitatively assess cell proliferation in various human fetal kidney cell populations during development.
  • To correlate changes in proliferation index (PI) with the differentiation stages of developing nephron segments.

Main Methods:

  • Utilized antibodies against PCNA to determine the proliferation index (PI) in histologically normal human fetal kidneys (10 weeks gestation to term).
  • Evaluated PI separately in immature mesenchyme (blastema), immature early tubules, ampulla of ureteric bud, proximal tubules, Tamm-Horsfall protein (THP)-positive tubules, distal tubules, collecting ducts, and glomeruli.

Related Experiment Videos

Main Results:

  • Immature early tubules maintained a high PI (33-43) throughout embryonic development.
  • Blastemal cell PI was initially high, decreasing from the second trimester.
  • PI of THP-positive tubules, distal tubules, collecting ducts, and glomeruli decreased significantly from early development to term.
  • Differentiated proximal tubules exhibited a consistently low PI (<1) regardless of gestational age.

Conclusions:

  • This is the first quantitative study detailing PI changes across human nephrogenesis.
  • Observed PI variations correlate directly with the differentiation status of specific developing nephron segments.
  • These findings provide critical insights into the cellular dynamics of human kidney development.