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Updated: Jul 19, 2026

A Method for Targeted 16S Sequencing of Human Milk Samples
Published on: March 23, 2018
HIV transmission through breastfeeding: a study in Malawi
P G Miotti1, T E Taha, N I Kumwenda
1Department of Epidemiology, Johns Hopkins School of Hygiene and Public Health, Baltimore, MD, USA. pm122m@nih.gov
Context:
Understanding the risk of human immunodeficiency virus (HIV) transmission through breastfeeding is essential for advising HIV-infected mothers and formulating public health policy recommendations.
Objective:
To measure the frequency, timing, and risk factors of HIV transmission through breast milk.
Design:
Prospective cohort study conducted between 1994 and 1997, with follow-up of infants through 24 months of age.
Setting:
Postnatal clinic of tertiary care hospital, Blantyre, Malawi.
Participants:
A total of 672 infants (HIV-negative at birth) born to HIV-infected women who had not received antiretroviral drugs during or after pregnancy.
Main Outcome Measure:
Incidence of HIV in breastfed infants by age and maternal and infant risk factors for HIV transmission, using proportional hazard models to derive risk ratios (RRs) and 95% confidence intervals (CIs).
Results:
Forty-seven children became HIV-infected while breastfeeding but none after breastfeeding had stopped. The cumulative infection rate while breastfeeding, from month 1 to the end of months 5, 11,17, and 23, was 3.5%, 7.0%, 8.9%, and 10.3%, respectively. Incidence per month was 0.7% during age 1 to 5 months, 0.6% during age 6 to 11 months, and 0.3% during age 12 to 17 months (P = .01 for trend). The only factors significantly associated with low risk of postnatal HIV transmission in a multivariate model were high maternal parity (RR, 0.23; 95% CI, 0.09-0.56) and older maternal age (RR, 0.44; 95% CI, 0.23-0.84).
Conclusions:
Our data suggest that the risk of HIV infection is highest in the early months of breastfeeding, which should be considered in formulating breastfeeding policy recommendations.
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