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Updated: Aug 15, 2026

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
Published on: September 14, 2014
Chemical synthesis of yeast mitochondrial ATP synthase membranous subunit 8
M Goetz1, J M Schmitter, S Geoffre
1Institut Europeén de Chimie et de Biologie, Ecole Polytechnique, Université Bordeaux I, Talence, France. michael.goetz@iecb-polytechnique.u-bordeaux.fr
Abstract:
Chemical synthesis of highly hydrophobic peptides and proteins remains a challenging problem. Strong interchain associations within the peptide-resin matrix have to be overcome. A synthetic strategy for solid phase peptide synthesis is proposed, mainly based on prolonged coupling time using aprotic polar solvent mixtures. A tailored chromatographic purification was required to obtain a sample sufficiently pure for structural analysis. In this work, the total chemical synthesis of the membrane-embedded yeast mitochondrial ATP synthase subunit 8 is described. The quality of the synthetic protein was checked by electrospray mass spectrometry, its tendency to adopt alpha-helical secondary structure is evidenced by circular dichroism spectroscopy.
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