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Isotope dilution high-performance liquid chromatography/tandem mass spectrometry method for quantifying urinary
M D Beeson1, W J Driskell, D B Barr
1National Center for Environmental Health, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia 30341, USA. mwb9@cdc.gov
Analytical Chemistry
|August 28, 1999
Abstract:
We have developed an isotope dilution high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method for quantifying the urinary metabolites of the pesticides atrazine, malathion, and 2,4-dichlorophenoxyacetic acid (2,4-D). Urine samples are extracted with an organic solvent, and the organic fraction is concentrated. The concentrate is then analyzed using HPLC/MS/MS. The limits of detection for the metabolites are less than 0.5 microgram/L (parts per billion) in 10 mL of urine, with a high degree of accuracy and precision.