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Isolation and characterization of simple sequence repeat loci in the gray tree frog, Hyla chrysoscelis
J D Krenz1, R D Semlitsch, H C Gerhardt
1Division of Biological Sciences, University of Missouri, Columbia 65211-7400, USA. john.krenz@mankato.msus.edu
Genome
|August 28, 1999
Summary
Researchers developed simple sequence repeat (SSR) markers for gray tree frogs (Hyla chrysoscelis). These SSR markers are valuable genetic tools for population studies and conservation efforts.
Area of Science:
- Genomics
- Molecular Biology
- Amphibian Genetics
Background:
- Simple sequence repeats (SSRs) are crucial genetic markers.
- Effective SSR markers are needed for population genetics in gray tree frogs (Hyla chrysoscelis).
Purpose of the Study:
- To construct and characterize a genomic library for Hyla chrysoscelis.
- To identify and assess the utility of simple sequence repeat (SSR) loci within this species.
Main Methods:
- Construction of a partial genomic library (approx. 10,000 clones, 350 bp inserts).
- Screening the library using six SSR repeat oligonucleotides (AC, AG, ACG, AGC, AAC, AAG).
- Sequence analysis of positive clones to identify SSR loci.
Main Results:
- Identified 31 unique positive clones containing 41 SSR loci.
- Dinucleotide repeats were more prevalent (36/41 loci) than trinucleotide repeats.
- AC and AG probes identified the majority of loci (26 and 7, respectively); AT repeats were also found.
Conclusions:
- The characterized SSR loci represent valuable genetic markers for Hyla chrysoscelis.
- These markers will facilitate studies on identity, parentage, and lineage in gray tree frogs.