Related Experiment Video

Updated: Oct 19, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Targeting nitric oxide to cancer cells: cytotoxicity studies of glyco-S-nitrosothiols

Y Hou1, J Wang, P R Andreana

  • 1Department of Chemistry, Wayne State University, Detroit, MI 48201, USA.

Bioorganic & Medicinal Chemistry Letters
|August 28, 1999
PubMed

Insights

New glyco-S-nitrosothiols, including fructose-2-SNAP and glucose-2-SNAP, show enhanced cytotoxicity against prostate cancer cells. These compounds are more effective than SNAP in killing DU-145 cells in vitro.

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Cancer Biology

Background:

  • S-nitrosothiols (SNPs) are compounds that release nitric oxide (NO), a signaling molecule with diverse biological roles.
  • Nitric oxide plays a role in cancer progression and cell death.
  • Developing novel SNP derivatives can offer new therapeutic strategies.

Purpose of the Study:

  • To synthesize novel glyco-S-nitrosothiols, specifically fructose-2-SNAP and glucose-2-SNAP.
  • To evaluate the in vitro cytotoxicity of these novel compounds against human prostate cancer cells.
  • To compare their efficacy with the parent compound, SNAP.

Main Methods:

  • Chemical synthesis of fructose-2-SNAP and glucose-2-SNAP.
  • In vitro cytotoxicity assays using DU-145 human prostate cancer cell line.
  • Comparison of cell viability after treatment with synthesized compounds and SNAP.

Main Results:

  • Fructose-2-SNAP and glucose-2-SNAP were successfully synthesized.
  • Both novel glyco-S-nitrosothiols demonstrated significantly higher cytotoxicity compared to SNAP.
  • The compounds effectively reduced the viability of DU-145 prostate cancer cells in vitro.

Conclusions:

  • Glyco-S-nitrosothiols represent a promising class of compounds for prostate cancer therapy.
  • Fructose-2-SNAP and glucose-2-SNAP exhibit superior cytotoxic effects against prostate cancer cells than SNAP.
  • Further investigation into the mechanisms and in vivo efficacy of these compounds is warranted.

More Related Videos

Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment04:48

Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

Published on: January 7, 2015

Analytical Techniques for Assaying Nitric Oxide Bioactivity11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...