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[Studies of foreign gene integeation during embryo early development].

Y F Lu1, C Tian, J X Deng

  • 1Institute of Biotechnology, Academy of Military Medical Science, Beijing.

Yi Chuan Xue Bao = Acta Genetica Sinica
|August 31, 1999
PubMed
Summary
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Identifying foreign gene integration in early-stage mouse embryos using PCR significantly improves transgenic animal production efficiency. This method screens for successful gene integration before implantation, reducing costs and increasing success rates.

Area of Science:

  • Animal biotechnology
  • Molecular biology
  • Genetics

Context:

  • Transgenic animal production faces challenges with gene transfer efficiency and cost.
  • Early identification of foreign gene integration is crucial for improving success rates.

Purpose:

  • To develop and evaluate a method for identifying foreign gene integration in mouse embryos.
  • To assess the efficiency of gene integration at different embryonic stages (1, 2, and 8 cells).

Summary:

  • Mice fertilized eggs were microinjected with a construct containing the whey acidic protein (WAP) gene promoter directing the G-CSF gene.
  • Polymerase Chain Reaction (PCR) was used to detect foreign gene retention and integration.
  • Retention rates were 100%, 77.7%, and 44.4% at 1, 2, and 8 cell stages, respectively.

Related Experiment Videos

  • Using homologous recombination fragments, integration rates were 11.1%, 55.5%, and 44.4% at 1, 2, and 8 cell stages, respectively.

    • Impact:

    • This technique offers a potential method for screening transgenic embryos in farm animals using embryo section techniques.
    • Improved screening can lead to more efficient and cost-effective production of transgenic livestock.