Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors.

H Mizuguchi1, M A Kay

  • 1Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan.

Human Gene Therapy
|August 31, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of physical activity on mother-child relationship and parental attitudes: a follow-up study examining the long-term effects of COVID-19.

European review for medical and pharmacological sciences·2023
Same author

A MicroRNA Derived from Adenovirus Virus-Associated RNAII Promotes Virus Infection via Posttranscriptional Gene Silencing.

Journal of virology·2018
Same author

A phase III trial comparing oral S-1/cisplatin and intravenous 5-fluorouracil/cisplatin in patients with untreated diffuse gastric cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2017
Same author

Efficacy of trifluridine and tipiracil (TAS-102) versus placebo, with supportive care, in a randomized, controlled trial of patients with metastatic colorectal cancer from Spain: results of a subgroup analysis of the phase 3 RECOURSE trial.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2016
Same author

NF-κB promotes leaky expression of adenovirus genes in a replication-incompetent adenovirus vector.

Scientific reports·2016
Same author

Activity levels of cathepsins B and L in tumor cells are a biomarker for efficacy of reovirus-mediated tumor cell killing.

Cancer gene therapy·2015
Same journal

Genome Editing for Familial Hemophagocytic Lymphohistiocytosis: Design Principles, Challenges, and Translational Perspectives.

Human gene therapy·2026
Same journal

Lilly, Ascidian Launch Up-to-$1.9B RNA Exon Editor Collaboration.

Human gene therapy·2026
Same journal

Hyperthermia-Enhanced Adeno-Associated Virus Gene Therapy: Mechanisms, Delivery Technologies, and Translational Potential.

Human gene therapy·2026
Same journal

Adeno-Associated Virus Gene Therapy Translation: Lessons from Early Regulatory Meetings.

Human gene therapy·2026
Same journal

In Remembrance of Professor Zelig Eshhar: <i>A Life Committed to CAR-T</i>.

Human gene therapy·2026
Same journal

<i>In Vivo</i> Genome Editing Approach to Disrupt Hydroxyacid Oxidase 1 for the Treatment of Primary Hyperoxaluria Type 1.

Human gene therapy·2026
See all related articles

Researchers simplified adenoviral vector construction using a two-plasmid in vitro ligation method. This improved system now allows for the production of E1/E4-deleted adenoviral vectors, enhancing gene therapy development.

Area of Science:

  • Molecular Biology
  • Virology
  • Gene Therapy

Background:

  • Adenoviral vectors are crucial tools in gene therapy and molecular biology.
  • Previous methods for creating recombinant adenoviral vectors, such as E1- or E1/E3-deleted types, often involved complex recombination steps.
  • A simplified in vitro ligation method was previously established to bypass recombination for E1- or E1/E3-deleted vector production.

Purpose of the Study:

  • To enhance the simplicity of adenoviral vector construction.
  • To adapt the existing two-plasmid in vitro ligation system.
  • To enable the production of E1/E4-deleted adenoviral vectors.

Main Methods:

  • Modification of a previously developed two-plasmid in vitro ligation system.
  • In vitro ligation techniques were employed to assemble adenoviral vector genomes.

Related Experiment Videos

  • The system was adapted to specifically target the deletion of E1 and E4 genes.

    • Main Results:

    • The modified system significantly improved the ease of vector construction.
    • Successful production of recombinant adenoviral vectors with E1/E4 deletions was achieved.
    • The method eliminated the need for a separate recombination step.

    Conclusions:

    • The refined two-plasmid in vitro ligation method offers a more straightforward approach to generating adenoviral vectors.
    • This advancement facilitates the production of E1/E4-deleted adenoviral vectors, expanding their utility in research and therapeutic applications.
    • The simplified process streamlines the development of novel adenoviral-based gene delivery systems.