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The selective increase or decrease of organellar DNA in generative cells just after pollen mitosis one controls

N Nagata1, C Saito, A Sakai

  • 1Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan. nagata@postman.riken.go.jp

Planta
|August 31, 1999
PubMed
Summary

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Organellar DNA presence in mature pollen determines inheritance patterns. Mitochondrial and plastid DNA in generative cells correlate with paternal/biparental or maternal inheritance, respectively, influencing organelle DNA content post-pollen mitosis.

Area of Science:

  • Plant Biology
  • Genetics
  • Cell Biology

Background:

  • Organellar inheritance in plants is crucial for understanding genetic transmission.
  • Mitochondria and plastids possess their own DNA, inherited through specific mechanisms.
  • Pollen development involves distinct cell types, including generative cells that contribute to inheritance.

Purpose of the Study:

  • To investigate the presence and distribution of organellar DNA (mitochondrial and plastid) in mature pollen generative cells of eight angiosperm species.
  • To correlate the observed organellar DNA presence with genetically determined modes of organellar inheritance (maternal, paternal, or biparental).
  • To understand the dynamics of organellar DNA content in generative cells following pollen mitosis.

Main Methods:

Related Experiment Videos

  • Fluorescence microscopy was employed to visualize organellar DNA.
  • Technovit 7100 resin embedding and double staining with DAPI and DiOC(6) were used for visualization.
  • Eight angiosperm species with known genetic modes of organellar inheritance were analyzed.

    • Main Results:

    • Species were classified into four types based on mitochondrial (m) and plastid (p) DNA presence in generative cells: m+p+, m+p-, m-p+, and m-p-.
    • The presence of organellar DNA in generative cells directly corresponded to paternal/biparental (for mitochondria) or maternal (for plastids) inheritance.
    • Organellar DNA content increased post-pollen mitosis when present, and decreased when absent in generative cells.

    Conclusions:

    • The presence or absence of mitochondrial and plastid DNA in mature generative cells dictates their mode of inheritance.
    • Mitochondrial and plastid inheritance are regulated independently in young generative cells after pollen mitosis.
    • This study provides a cytological basis for understanding diverse organellar inheritance patterns in angiosperms.