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Related Experiment Videos

Rhes: A striatal-specific Ras homolog related to Dexras1.

J D Falk1, P Vargiu, P E Foye

  • 1Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

Journal of Neuroscience Research
|September 1, 1999
PubMed
Summary

Researchers identified a novel Ras-like GTP-binding protein, Ras homolog enriched in striatum (Rhes), predominantly in the rat brain's striatum. Its expression is regulated by thyroid hormone, suggesting a role in striatal development and function.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • The striatum is a key brain region involved in motor control and reward.
  • Signal transduction cascades are crucial for neuronal function and development.
  • Ras-like GTP-binding proteins are important regulators of cellular processes.

Purpose of the Study:

  • To characterize a novel mRNA (SE6C) predominantly expressed in the rat striatum.
  • To identify and analyze the protein product of SE6C, named Ras homolog enriched in striatum (Rhes).
  • To investigate the functional and regulatory properties of Rhes.

Main Methods:

  • Subtractive hybridization to identify novel mRNAs.
  • cDNA cloning and sequencing to determine the full-length transcript.

Related Experiment Videos

  • Bioinformatic analysis to compare Rhes with known proteins.
  • Bacterial expression and GTP-binding assays to confirm GTPase activity.
  • Hormonal induction studies to investigate gene regulation.

    • Main Results:

    • A full-length cDNA for rat SE6C mRNA was characterized, encoding a 266 amino acid protein, Rhes.
    • Rhes shares significant similarity with Ras-like GTP-binding proteins, particularly Dexras1, but possesses a unique C-terminus.
    • Human Rhes homolog identified on chromosome 22q13.1 shares 95% identity with rat Rhes.
    • Bacterially expressed Rhes demonstrates GTP-binding and GTPase functionality.
    • Rhes expression is dependent on thyroid hormone availability and shows postnatal accumulation, but is not induced by dexamethasone.

    Conclusions:

    • Rhes is a novel Ras-like GTP-binding protein enriched in the striatum.
    • The unique C-terminus of Rhes suggests specialized functions compared to other Ras-like proteins.
    • Thyroid hormone regulates Rhes expression, indicating a role in postnatal striatal development.
    • Rhes is a component of signal transduction cascades within the striatum.