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Stability and compatibility of tacrolimus and fluconazole in 0.9% sodium chloride
1College of Pharmacy, University of Michigan, Ann Arbor 48109-1065, USA. cejohn@umich.edu
Objective:
To determine the physical compatibility and chemical stability of tacrolimus injection and fluconazole injection combined in a range of concentrations used clinically for intravenous Y-site co-administration over 3 hours.
Design:
Tacrolimus injection (Prograf) was combined with fluconazole injection (Diflucan) in volumetric glass flasks at room temperature (23 degrees C to 25 degrees C) to form final concentrations of 5 or 20 micrograms/mL and 500 or 1,500 micrograms/mL, respectively. Immediately after preparation and at 1.5 and 3 hours, triplicate samples and controls were visually inspected and assayed in duplicate by high-performance liquid chromatography; pH of the samples was also determined.
Setting:
University of Michigan College of Pharmacy.
Patients:
Not applicable.
Interventions:
Not applicable.
Main Outcome Measures:
Initial and subsequent concentrations of tacrolimus and fluconazole at 1.5 and 3 hours.
Results:
More than 99% of the initial concentration of each drug remained in all samples throughout the study period. There was no color change, and the pH of the samples did not change appreciably.
Conclusion:
Tacrolimus and fluconazole are stable and compatible in a range of clinically used concentrations for 3 hours and can be intravenously co-administered through a Y-site.