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Related Experiment Videos

Sonoclot coagulation analysis: a study of test variability.

G Ekbäck1, O Carlsson, U Schött

  • 1Department of Anesthesiology and Intensive Care, Orebro Medical Center Hospital, University of Orebro, Sweden.

Journal of Cardiothoracic and Vascular Anesthesia
|September 1, 1999
PubMed
Summary

Reproducibility of Sonoclot coagulation analysis (SCA) improved by optimizing activators, using native blood, and performing repeated analyses. These changes significantly reduced high variability in SCA parameters.

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Area of Science:

  • Clinical Pathology
  • Hematology
  • Medical Device Analysis

Background:

  • Sonoclot coagulation analysis (SCA) has shown high variation coefficients for coagulation parameters.
  • The preloaded celite activator in standard Sono-cuvettes may contribute to this variability.
  • The optimal blood sample type for SCA (citrated vs. native whole blood) remains unclear.

Purpose of the Study:

  • To assess the reproducibility of Sonoclot coagulation analysis (SCA).
  • To investigate if the celite activator mix/release in Sono-cuvettes causes high SCA parameter variability.
  • To determine if citrated whole blood is optimal for SCA.

Main Methods:

  • Prospective trial conducted at an academic teaching medical center.
  • Eight healthy volunteers underwent repeated blood sampling via indwelling radial artery catheters.

Related Experiment Videos

  • Seven Sonoclot analyzers were used to test various activators (types, amounts, forms) and blood types (citrated vs. native).

    • Main Results:

    • High variance (SDs up to 200% of median) was confirmed for SCA with citrated blood and standard cuvettes.
    • Using native blood and/or alternative activators like kaolin significantly reduced SCA variance (p < 0.05).
    • Performing repeated Sonoclot analyses further decreased variance to 10%-35% of single-analysis variance.

    Conclusions:

    • Optimizing Sonocuvette activators, employing native whole blood, and conducting repeated analyses significantly enhance SCA reproducibility.
    • These modifications effectively reduce the previously documented high variability associated with the Sonoclot instrument.
    • Findings suggest improvements for more reliable coagulation assessments using SCA.