Trigeminal innervation of the mammalian pineal gland

There is evidence that the trigeminal (Gasserian) ganglia innervate the mammalian pineal gland and serve in its regulation in addition to the sympathetic and cholinergic as well as further influences. By means of immunohistochemical methods, previous studies demonstrated fibers containing calcitonin gene-related peptide (CGRP-LI) or substance P (SP) in the superficial pineal of various mammalian species. In addition, SP and the related tachykinin, neurokinin A, were detected by radioimmunoassay and HPLC, respectively, in the rat and human pineal gland. In the present study, retrograde neuronal tracing upon injection of a tracer substance into the superficial pineal gland of rats was used in combination with immunohistochemistry to show that trigeminal ganglionic neurons innervate the gland, and that a considerable amount of these neurons are also CGRP- or SP-immunoreactive. These results reveal that afferent neuropeptidergic entering the superficial pineal gland originate from the trigeminal ganglia. The present paper reviews the evidence for a CGRP- and SP-ergic innervation of the mammalian pineal gland and discusses the possible role of these neuropeptides with regard to pineal function.