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Improved method to measure urinary alkoxyacetic acids.

T S Shih1, J S Chou, C Y Chen

  • 1Institute of Occupational Safety and Health, Council of Labor Affairs, Taipei, Taiwan, Republic of China. stone@mail.cla.gov.tw

Occupational and Environmental Medicine
|September 3, 1999
PubMed
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This study introduces a simplified, reliable gas chromatography/mass spectrometry (GC/MS) method for measuring urinary alkoxyacetic acids. The new method enhances the biological monitoring of occupational exposure to ethylene glycol ethers.

Area of Science:

  • Analytical Chemistry
  • Occupational Health
  • Environmental Science

Background:

  • Conventional methods for measuring urinary alkoxyacetic acids can be complex and lack specificity.
  • Ethylene glycol ethers are widely used, and monitoring occupational exposure is crucial for worker safety.

Purpose of the Study:

  • To develop a simplified, more specific, and reliable analytical method for quantifying urinary alkoxyacetic acids.
  • To improve the biological monitoring of occupational exposure to ethylene glycol ethers.

Main Methods:

  • Urine samples were acidified and extracted using a mixed solvent of methylene chloride and isopropyl alcohol.
  • Analysis was performed using gas chromatography/mass spectrometry (GC/MS) without derivatization.
  • Optimal extraction conditions (pH, solvent ratio, time) were determined.

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Main Results:

  • The optimized method demonstrated high precision (pooled coefficient of variation < 6.41%) and sensitivity (low limits of detection).
  • Urine samples showed stability for at least 5 months and 3 freeze-thaw cycles.
  • The new GC/MS method showed excellent correlation with a conventional method for methoxyacetic acid (MAA) in exposed workers.

Conclusions:

  • The simplified, non-derivatization GC/MS method is specific, sensitive, simple, safe, and reliable.
  • This method is suitable for the biological monitoring of occupational exposure to ethylene glycol ethers.
  • The improved method facilitates accurate assessment of worker exposure levels.