Related Experiment Videos
[Resistance patterns of ovine mastitis pathogens]
P Winter1, A Höflechner, W Baumgartner
1II. Medizinischen Universitätsklinik für Klauentiere, Veterinärmedizinischen Universität Wien. petra.winter@vu.wien.ac.at
Abstract:
For control of the udder health status of milk sheep the minimal inhibitory concentration of mastitis causing pathogens to 12 different chemotherapeutics was determined by means of Sensititre microplate method. 73 staphylococci- and 8 streptococci-strains was examined. Following staphylococci-strains were found: S. aureus (11), S. epidermidis (34), S. chromogenes and S. xylosus (6 each), S. hyicus and S. warneri (each 5) as well as S. sciuri and S. simulans (3 each). MIC-values to penicillin, amoxycillin and ampicillin of 2 penicillinase-positive S. aureus-strains were more than 4.0 micrograms/ml. The remaining isolates and the major part of coagulase-negative staphylococci displayed MIC-values up to 1.0 microgram/ml to beta-lactamantibiotics except cloxacillin. Against cephalosporines, cephalexin excluded, and aminoglycosides very low MIC-values concerning staphylococci were observed. In case of spiramycin MIC-levels more or less than 8.0 micrograms/ml were determined. Streptococci exhibited MIC-values in the range of 0.06 to 0.5 microgram/ml against beta-lactamantibiotics with exclusion of cloxacillin, cefoperazone and cefquinome and spiramycin as well.
Insights
Minimal inhibitory concentrations of mastitis pathogens in milk sheep were determined. Most staphylococci and streptococci strains showed susceptibility to various antibiotics, guiding udder health management strategies.
Area of Science:
- Veterinary Microbiology
- Antimicrobial Resistance
- Sheep Health
Context:
- Mastitis is a significant concern in milk sheep production, impacting udder health and economic viability.
- Accurate susceptibility data for mastitis-causing pathogens is crucial for effective treatment protocols.
Purpose:
- To determine the minimal inhibitory concentration (MIC) of 12 chemotherapeutics against mastitis pathogens in milk sheep.
- To evaluate the efficacy of various antibiotics against prevalent staphylococci and streptococci strains.
Summary:
- The study analyzed 73 staphylococci and 8 streptococci strains using the Sensititre microplate method.
- Penicillinase-positive Staphylococcus aureus strains showed high resistance to penicillin, amoxicillin, and ampicillin.
- Most staphylococci, including coagulase-negative strains, were susceptible to beta-lactam antibiotics (except cloxacillin), cephalosporins, and aminoglycosides.
- Streptococci demonstrated broad susceptibility to beta-lactam antibiotics (excluding specific agents) and spiramycin.
Impact:
- Provides essential data for selecting appropriate antibiotic treatments for mastitis in milk sheep.
- Informs veterinary decisions regarding udder health management and antimicrobial stewardship in sheep farming.
- Highlights the need for ongoing surveillance of antimicrobial resistance in mastitis pathogens.