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Transformation in Bacillus subtilis with nitrogen mustard crosslinked DNA. Effect on cotransformation and mutation
Biochimica Et Biophysica Acta
|December 21, 1978
Abstract:
Bacillus subtilis DNA was treated in vitro with nitrogen mustard and the crosslinked molecules were purified, after alkali denaturation, by hydroxyapatite chromatography. When tested for the ability to transform the trpC2-hisB2 segment, these molecules exhibited a decrease in the cotransformation index (r) as compared to native or renatured DNA. The decrease in r was not accompanied by an increase in mutagenicity.