Effect of Cryptotympana atrata extract on compound 48/80-induced anaphylactic reactions

We studied the effect of the aqueous extract of Cryptotympana atrata Fabricius on anaphylactic reactions. Cryptotympana atrata completely inhibited compound 48/80-induced systemic anaphylactic shock at doses of 0.5 and 1.0 g/kg. When Cryptotympana atrata was given as pretreatment at concentrations ranging from 0.025 to 1.0 g/kg, the plasma histamine levels induced by compound 48/80 were reduced in a dose-dependent manner. Cryptotympana atrata also inhibited histamine release from rat peritoneal mast cells (RPMC) by compound 48/80. In addition, when Cryptotympana atrata was added, the level of cAMP in RPMC transiently and significantly increased about 14-fold compared with that of control cells. Our studies provide evidence that Cryptotympana atrata may be beneficial in the treatment of nonspecific anaphylactic reactions.