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Temperature-dependent fermentation of D-sorbitol in Escherichia coli O157:H7
O M Bouvet1, S Pernoud, P A Grimont
1Unité des Entérobactéries, Unité INSERM 389, Institut Pasteur, 75724 Paris Cedex 15, France. obouvet@pasteur.fr
Applied and Environmental Microbiology
|September 3, 1999
Abstract:
The influence of growth temperature on the ability to ferment D-sorbitol was investigated in Escherichia coli O157:H7. It was found that O157:H7 strains have a temperature-sensitive sorbitol phenotype. D-Sorbitol transport and sorbitol-6-phosphate dehydrogenase activities were expressed in sorbitol-fermenting cells grown at 30 degrees C but only at a low level at 40 degrees C. Sorbitol-positive variants able to transport D-sorbitol were easily selected at 30 degrees C from culture of Sor(-) E. coli O157:H7 strains.